The Chinchilla Scientific Easy (1 – Reagent) Nitrate Method offers a suitable non-
hazardous alternative to traditional nitrate methods for aqueous solutions. The method
was primarily designed to be performed by automated discrete analysis, but can be
performed on most continuous flow analyzers or manually.
The procedure for determining nitrate utilizes a reaction in which nitrate is reduced to
nitrite by a proprietary reagent “R1”. The reduced nitrate sample reacts under acidic
conditions with the reagent and forms a highly colored soluble dye, which is measure
colorimetric ally as nitrite between 520and 550 nm. The final product measured
represents the Nitrite ion originally present, plus that formed from the reduction of nitrate
(Nitrate+Nitrite). The method is available at no charge for Chinchilla Scientific discrete
analyzer customers or can be licensed for a small one time charge.
May 18, 2012 Final Method Update rule lists Chinchilla Scientific Easy (1– Reagent)
Nitrate Method as an approved method for nitrate, and nitrite combined nitrate nitrite
analysis under 40 CFR Part 136.3. The USEPA determined that the method meets all
requirements at 40 CFR Part 136 for measurement of nitrate and nitrite singly or
combined in water and wastewater.
The method also received approval on August 3, 2009 (Federal Register / Vol. 74, No.
147 / Rules and Regulations) for drinking water compliance monitoring. The USEPA
determined that the methods is “equally effective” as current Safe Water Drinking Act
(SWDA) methods for nitrate. The new method meets all requirements at 40 CFR Part
141 for measurement of nitrate and nitrite singly or combined in drinking water.